The subway protection and sealing partition device relates to the protective isolation technology of underground engineering, especially suitable for large-scale rail transit in underground and emergency situations for the protection and isolation of rail transit passages. The product consists of an upper sliding plate part, a lower sliding plate part, a door leaf part, a door frame part, a hinge part, a locking part, etc., without the need for a dedicated hidden space, saving construction investment and reducing subway costs. The device uses semi-axle hinges and jacks, making the opening and closing of the door leaf flexible and light, with a quick opening and closing speed, meeting the requirements for rapid transition between emergency and normal states. It employs a manual structure composed of worm gear transmission mechanism, screw nut transmission mechanism, and high pair rotating mechanism, which can simultaneously control the movement of the upper sliding plate mechanism, lower sliding plate mechanism, and locking mechanism, allowing the protective partition device to be closed, sealed, or opened at the fastest speed. In emergency situations, this patent's upper and The lower sealing plate and the upper plug board fix and seal the cables inside the channel without damaging them. Once the emergency state is lifted, the entire subway can be put into use immediately.