This invention discloses a method for preparing lymph dye carriers using silica nanoparticles, which belongs to the intersection of micro-nano technology and the foundation of clinical medicine. The invention connects dyes that mark lymph nodes to the surface, pores, or internal cavities of silica nanoparticles through adsorption, embedding, and other methods, forming silica nanoparticles that can be used for marking and identifying sentinel lymph nodes. This invention has substantial characteristics and significant progress. It can retain the dye in the sentinel lymph node by the steric effect of the spatial volume of silica or slow down the flow rate passing through the sentinel lymph node. In addition, it can also use the lymphatic targeting characteristic of nano-silica to enable targeted release or targeted sustained release of the dye into the lymph nodes. The silica nanoparticles as carriers for the lymph dye provided by this invention can improve the convenience and accuracy of locating sentinel lymph nodes. It has important scientific research value and broad application prospects in the field of general tumor surgery.