Biological valves have been used in clinical practice for decades, but the supply of homografts is limited. Heterografts are ideal substitutes, but years of clinical use have revealed their tendency to degenerate within the body, thereby limiting their widespread application. This study uses a self-developed heterologous (pig) stentless aortic valve as the scaffold, and applies tissue engineering methods to seed animal endothelial cells onto it, forming a cell-active heterologous biological valve. Subsequently, an animal implantation experiment was conducted, with six sheep of six months old each being implanted with either a tissue-engineered heterologous stentless biological valve or a non-tissue-engineered valve. Ultrasound observations were made on the function of the implanted valves at 1, 2, and 3 months postoperatively. After three months, the valves were removed for scanning electron microscopy, isotope H-adenine deoxyribonucleoside uptake rate, pathology, and functional testing. The structural and functional characteristics of the valves treated by the two methods were compared. Differences in mechanical properties and cell survival. The research results indicate that the pig aortic valve as a source of heart valve stent is a reliable and structurally reasonable substitute. Glutaraldehyde-treated heterograft valves, after being treated with L-glutamic acid, can successfully eliminate the cytotoxicity of glutaraldehyde and can successfully implant endothelial cells. Endothelial cells seeded on the valve can reduce immune reactions, prevent thrombus formation on the valve, decrease calcium salt deposition on the valve, reduce calcification of the valve, thereby delaying valve degeneration. Tissue engineering valves have unparalleled durability compared to non-tissue valves. This study has developed a new type of anti-degradation tissue engineering heterograft stented bioprosthesis, which has laid the foundation for further research and clinical application through animal experiments, and has significant practical value. Experts discuss that this study has reached the leading level domestically.