This project belongs to the field of integrated traditional Chinese and Western medicine internal science. The main content includes conducting research on drug interactions between traditional Chinese medicine (TCM) and Western medicine based on the P450 enzyme system and transporter systems, resulting in the following innovative achievements: 1. Integrating the team's strengths, establishing and optimizing a first-class domestic platform for evaluating metabolic interactions of TCM, and completing the construction of a database on the relative activity of more than 30 kinds of TCM and over 100 monomers on the P450 enzyme system; 2. Actively leveraging clinical advantages, conducting intervention studies on lipid-lowering formulas based on patient's lipid levels and risk factor stratification. Clinical research found that when combined with atorvastatin, the lipid-lowering formula can effectively reduce patients' TC and LDL levels, and the combination is significantly more effective than monotherapy, indicating a certain synergistic interaction between the two; 3. No matter how complex the components of traditional Chinese medicine are, The material basis that functions within the body still belongs to the category of compounds. Previous studies based on transporters (OATP1B1/BCRP/MDR1) and the CYP450 enzyme system have confirmed that traditional Chinese medicine lipid-lowering formulas have varying degrees of inhibitory effects on transporters and CYP3A4; 4. Further research has proven that both the traditional Chinese medicine lipid-lowering formula and its components have strong inhibitory effects on CYP3A4, which can effectively slow down the metabolism rate of atorvastatin in the liver microsomal environment. Pharmacokinetic studies in healthy volunteers have shown that adding the lipid-lowering formula significantly delays the metabolism rate of atorvastatin in the body, increasing its bioavailability. Therefore, there is a drug interaction between traditional Chinese medicine and Western medicine through the P450 enzyme system; 5. Through multiple clinical trials, it has been verified that there is a certain synergistic effect between traditional Chinese medicine and Western medicine, and safety assessments have been conducted. At the same time, we conducted a promotion and application research, which showed good promotability; Project characteristics: The combined use of traditional Chinese medicine (TCM) and Western medicine is currently a commonly used clinical treatment method, which effectively improves clinical efficacy and quality of life. Drug-drug interactions (DDI) can occur through the inhibition or induction of drug metabolizing enzymes (including cytochrome P450 enzyme systems, etc.) and transporters, thereby causing the plasma and tissue concentrations of drugs and their metabolites to increase or decrease geometrically. Among them, through synergistic effects, the metabolism of active drugs is slowed down, increasing their blood drug concentration and bioavailability, but this also brings safety issues. In recent years, we have been committed to exploring the interactions between TCM and Western medicine based on the hepatic drug enzyme P450 enzyme system, glucuronosyltransferase metabolism system, and transporter system. Integrated team strengths, established and optimized the first-class domestic platform for evaluating the metabolic interactions of traditional Chinese medicine (TCM), completed the construction of a database on the relative activity effects of more than 30 TCM herbs and over 100 monomers on P450 enzyme systems, built a database on adverse reactions caused by the combined use of commonly used clinical TCMs with Western medicines, and further demonstrated that both the lipid-lowering formula of TCM and its components have strong CYP3A4/transporter inhibitory effects. In both liver microsomal environments and healthy volunteers, it was proven that the TCM lipid-lowering formula can effectively slow down the metabolism rate of atorvastatin, verifying the existence of drug interactions between TCM and Western medicines. This scientific research work not only allows for a deeper exploration of the compatibility mechanisms of TCM but also better guides the clinical combination of drugs and monitors adverse reactions caused by drug interactions. Application and Promotion: The lipid-lowering formula is an empirical prescription from our department. Having been clinically applied for over a decade, it has proven to be effective and has been widely used in medical institutions such as Longhua Hospital, Yueyang Hospital, and the Municipal Traditional Chinese Medicine Hospital. In 2012 and 2013, reports on related topics were presented at the Oriental International Cardiology Conference and the Southern International Cardiology Conference, respectively, attracting considerable attention in the field. The achievements involved in this project include: training 6 graduate students, publishing 10 papers, obtaining 1 patent, and having articles cited 22 times.